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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70109-923-15B
中文名称:
英文名称:BOTTLE MEDIA PETG 500ML 40/CS
货期:热销品现货,其他请咨询




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文献和实验Yeast Media, Solutions and Stocks
, then add 50 ml of sterile 40% glucose 6.7 g yeast nitrogen base (without amino acids) Difco# 0919-15 SD plates Add 20 g Bacto-agar before autoclaving Sorbitol-Agar Medium (1 liter) 182 g sorbitol 6.7 g
for 20 minutes in 500 ml bottles in the RC5-B using the GS3 rotor. Resuspend the cell pellets in old media and transfer to two bottles, centrifuge as before, and decant the media. The cell pellets can be frozen at -70degC at this point. 3. Resuspend
, into a sterile 15ml conical centrifuge tube. V. Reagents: Chang media: IRVINE cat# T100-018, liquid and frozen supplement,100ml bottle, store supplement frozen, basal media at 4° C. Colcemid: GIBCO cat# 15210-040. 10µg/ml in Hank's
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