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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310384931
中文名称:
英文名称:X25 Membrane Vivaspin 2 0.4-2ml samples MWCO 5,000 Polyethersulfone (pack of 25)
货期:热销品现货,其他请咨询




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文献和实验. Incubate tubes on ice for 15 min. 3. Clear cell lysate by centrifuging samples at 18,000× g in arefrigerated tabletop microcentrifuge for 15 min at 4°C. Transfer supernatant to a fresh microcentrifuge tube. 4. Measure proteins concentration
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
) Polyethylenimine (PEI), branched, average mol. wt. ∼25,000 by LS (Sigma, cat. no. 408727‐100 ml) OPTI‐MEM reduced serum medium with L‐glutamine (Invitrogen, cat
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