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- 文献和实验
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- 库存:
999
- 供应商:
北京泽平
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EA
货号:T_70112-146-2
中文名称:
英文名称:MELTING POINT TUBES 150/VIAL
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文献和实验Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh
A Method for Micro-Scale Isolation and Purification of Gangliosides
. chloroform). 3. FIRST EXTRACTION Extract for 18 hr. at 4°C with magnetic stirring. 4. Centrifuge tubes at 2000 rpm (750 x g for 10 min.). carefully decant the completely clear supernate, without transferring any particulate matter, and process
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