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货号:T_701BASE20-G
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英文名称:SPEX BASE OIL 20 3.78 LTR 1GAL
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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
14. 50× TAE stock solution: 0.242 g/L Tris Base, 5.7% glacialacetic acid, 50 mM EDTA (pH 8.0). 15. 100-bp DNA ladder (Invitrogen). Store at -20°C. 16. GeneAmp PCR system 9700 (PE Applied Biosystem). 2.3. SplinkBlunt-PCR
Isolation of Retroelement from Plant Genomic DNA
for SSAP, REMAP and IRAP BARE-1 5' CTAGGGCATAATTCCAACAA. This corresponds to the first 19 bases of the BARE-1 LTR, facing outwards from the 5' LTR, plus one selective A base to inhibit internal priming within the BARE-1 element from the 3' LTR (see Waugh
LINEAGE ANALYSIS USING RETROVIRAL VECTORS
, we place a drop of mineral oil (e.g. a fresh tube of PCR oil) on top of the stock to prevent dehydration.REPLICATION-COMPETENT HELPER VIRUSReplication-competent virus is sometimes referred to as helper virus as it can complement ("help") a replication
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