赛默飞世尔Thermo Fisher X6 NEEDLE L

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

When you are ready to cast the fibrin gel construct, prep a 1 mL syringe with a 18G 1 ½ inch long needle. Have a 21G 1 inch needle ready as well.    4)      The fibrin solution is created at a 4:1:1 ratio of F solution: T solution: cell solution. To make one mL

PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS

Grip 35 x 10 mm, Falcon 3001-3);  KSOM medium;  light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);  aggregation (darning) needle ( DN-09, BLS Ltd., Hungary ,)  70% ethanol.  Method:  1. Place few rows