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999
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北京泽平
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货号：T_701AA30078DR
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英文名称：AL23 TUBE (1 END CLSD 18 200MM
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文献和实验- normally blunt-end ligation of insert to vector does not reform the restriction site (therefore when you design the oligo that make sure it doesn't reform the restriction site when ligated), therefore only religated vector is digested. See one-tube PCR
the restriction enzymes well. g) Keep the buffer in small aliquots as the freezing/defrosting cycles may damage the ATP. Also do not use too high a concentration of ATP (max. 0.5mM) for blunt-end ligation as polyadenation of blunt-ended may occur? h) The ligase
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
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