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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
A quick RNA mini-prep for Neurospora mycelial cultures
in aluminum foil and freeze in dry ice. Do not freeze in EtOH/dry ice bath because alcohol might seep through foil. Pads can be stored at -70°C for at least three weeks. 3. Wash a mortar and pestle thoroughly with warm water and Alconox (Fisher Scientific
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