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文献和实验Procedures for In Vitro Production of Bovine Embryos
hood 100 ml pipet tips and pipettor OMM + supplements Mineral oil 60 x 15 petri dishes Plastic pasteur pipet 3-4 bottles of OCM + supplement (warmed @ 38.5°C) 1 L saline + Pen/Strep (warmed @ 38.5°C)Preparation for Oocyte Collection Scalpel Petri dishes
SSR GEL and Silver Staining Protocol
μl 10% ammonium persulfate (0.1 g + 1 ml d.i. water) to the acrylamide mix in a beaker, gently mixing well. Then add 50 μl TEMED and mix. Polymerization will not start until TEMED has been added. Do not mix the catalysts together before adding
SSR GEL and Silver Staining Protocol
to first direction, don't use too much pressure. This treatment needs to be repeated every time. III. GEL SOLUTION PREPARATION: 1. Rinse all glassware and plastic ware with d.i. water prior to gel solution preparation and pouring, including
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