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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310474415
中文名称:
英文名称:X12 Filter unit Nalgene MF75 sterile 0.1µm pore steril VE=12 Stück
货期:热销品现货,其他请咨询




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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Tris(PH=6.8) 10% SDS 40 µl 10% ammonium persulfate 40 µl TEMED 7 µl C. Preparation of gel 1. Assemble the glass plates and spacers (0.75 mm thick). 2. Pour the running gel to about 1 cm below the wells of the comb (~3.2 ml). 3. Seal with 1 ml
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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