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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_701ASACE92X
中文名称：
英文名称：ACETATE STANDARD
货期：热销品现货，其他请咨询
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文献和实验: "Quickie" PCR is quite feasible: eg, [94oC 30 sec / 45oC 30 sec / 72oC 30 sec] x 30, for short products (200 - 500 bp). YOU CAN USE GLYCEROL IN THERMAL CYCLER REACTION TUBE HOLES TO ENSURE GOOD THERMAL CONTACTS DON'T RUN TOO MANY CYCLES: if you don't
Replication timing by density transfer
C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered
AFLP: not only for fingerprinting, but for positional cloning
2s 2) Precipitate the PCR products. Adjust the rxn products to 0.3 M Na-acetate and add 2.5 vol EtOH, incubate @ -20° C for _ 20 min, then spin @ 12K x g for 5 min. Resuspend each DNA in just enough 2x formamide buffer to load into 1 lane on a standard PA-AFLP gel
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