赛默飞世尔Thermo Fisher CHLORIDE 10

Measurement of Chloride Movement in Neuronal Preparations

; see protocol 3 , step ) or cultured cells (in 4‐ml quartz cuvette; see protocol 5 , step ) Low‐Cl− buffer supplemented with varying NaCl and gluconate (see recipe

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

of 0.125M and continue to rock or spin at room temp for 5 minutes;Next, pour off media and rinse plates with cold 1X PBS+1/1000 PMSF, aspirate and then add 1.5ml of PBS+1/1000 PMSF and scrape the plates to remove the cells. Collect the cultures

Clone Genes From a Phage Library

: This is a stabilization and storage solution for phage. 2.9g NaCl(50 mM), 1g Mg SO4(10 mM), 25 ml Tris pH 7.5 (50 mM), 50 mg gelatin (100 g/l) in 500 ml-- autoclave. • NaOH for plaque lifts: 0.5M NaOH, 1.5M Na Cl, 1 mM EDTA. • Tris for plaque lifts: 0.5M Tris HCl pH