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999
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英文名称：HI TEMP/LOW BLEED SEPTA 50/PK
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文献和实验Polyclonal and Monoclonal Antibodies
1) After collecting, blood should be allowed to clot for 60 min . at 37 C or O.N at 4 C. 2) Separate the clot from the sides of the tube (ringing) using a pasteur pipette. Place clot at 4 C O.N. 3) Spin at 10,000 x g for 10 min. at 4 C to separate
Monoclonal Antibody Production Protocol
buffer. (ex: HRP is 2000X). Leave 1 hr at room temp or O.N at 4°C. SUBSTRATE Dissolve substrate in water. Wash plate 4 times with PBS + 0.1% Tween 20 (use TBS instead of PBS for AP). Add 100 ul of substrate to every well. Match color development
. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). Leave 1 hr at room temp or O.N at 4°C. SUBSTRATE Dissolve substrate in water. Wash plate 4 times with PBS + 0.1 Tween 20 (use TBS instead of PBS
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