赛默飞世尔Thermo Fisher VIAL MICRO-

v468 chapter 5 糖原合成酶激酶-3 的抑制

for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin

Northern Blotting方法(Howell Lab)

inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube

Polyclonal and Monoclonal Antibodies

IMDM IMDM complete + 15% MCM. PBS PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 Aminopterin: Sigma, A 5159 (50X ) 1) Prepare a T-150 flask with 170 ml of IMDM complete with MCM instead of PCM