赛默飞世尔Thermo Fisher CHEMWARE TE

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

单细胞基因表达分析

for labeling buffer (Cat. No. BP328-1), 25-ml pipets for make-shift size-exclusion chromatography columns (Cat. No. 13-674-41E), and Pasteur pipettes (Cat. No. 13-678-20D) for filling the columns were from Fisher. Sephadex G-50 resin (Cat. No. G-50-150

Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH)

TEAB via glass Pasteur pipet into the prepared 25 ml pipet/column until liquid level is about a third of the way up. Add the G-50 suspension in the same manner and, as the matrix settles, remove the 1.5 ml tube “cap” to allow the matrix to settle