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货号：T_70115-236-1E
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
cliPS . 10% Denaturing Acrylamide gel, 100ml (for 20x40 cm glass plates 1.5mm thick) Swirl and warm up to 42C to allow for rapid polymerization (Heating is optional and not suggested for first-timers ) Pour gel: Add 678 μl
【转贴】immunofluorescence staining
the loose cells, pipetting solutions off manually will help.Part 2: ImmunostainingBlock with 5% powdered milk in TBS-T buffer plus 0.02% sodium azide (commonly called Blotto). Blocking time can be 30-60 min, or overnight at 4C. 3 percent serum from the 2nd
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