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999
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北京泽平
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EA
货号:T_70106-450-29
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英文名称:CAP RUBR LNR 18-415 500/CS
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文献和实验Procedures for In Vitro Production of Bovine Embryos
in 25 ml (4-7 x 106 /ml). To do so, add 10 ml sperm suspension to 90 ml water to kill sperm. Load 10 ml of sample onto a hemacytometer. Count the number of sperm in 5 squares (Figure 18) and multiply sperm number by 500,000 to determine concentration
PHEM (500 mls) 2x 18.14 g Pipes 6.5 g Hepes 3.8 g EGTA 0.99 g MgSO4 pH 7.0 w/ KOH PBS (5x in 500 mls) 20.45 g NaCl 0.465 g KCl 10.142 g Na2 HPO4 *7 H2 O 0.545 g KH2 PO4 pH 7.2 Mounting Media 20mM Tris pH
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
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