赛默飞世尔Thermo Fisher HISTOPLEX U

【转贴】immunofluorescence staining

m l buffer for 24 well trays, 1 ml for 6 well trays, etc.) Remove formaldehyde fix, discard in toxic waste bottle, and wash X 3 with PEM buffer, about 2-5 min. per wash.Incubate 5-30 min. with PEM + 0.5% Triton X-100. This step permeablizes the cells

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) Chloroform (CHCl 3 , Fisher) Acetic acid (AcOH, Acros) Silica gel (60 Å, 230 to 400 mesh, Merck

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

Toluene (Acros) Methylene chloride, anhydrous (CH 2 CH 2 , Fisher) Silica gel (60 Å, 230‐400 mesh; Merck