赛默飞世尔Thermo Fisher X25 NMR CAP

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Synthesis of Oligodeoxynucleotides with 5′‐Caps Binding RNA Targets

., Seneviratne, P.A., Allawi, H.T., and SantaLucia, J. Jr. 1999. Nearest‐neighbor thermodynamics and NMR of DNA sequences with internal A.A, C.C, G.G, and T.T mismatches. Biochemistry 38:3468‐3477.   

Stripping Western Blots

can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix