赛默飞世尔Thermo Fisher WHIRL-PAK S

LINEAGE ANALYSIS USING RETROVIRAL VECTORS

observed in vivo. Directly scoring histochemically stained, non-G418 selected NIH 3T3 cells led to an underestimate of the number of BAG infected colonies, presumably as such cells often are only faint blue, while DAP-infected cells are usually an intense

Stripping Western Blots

, harvest 24-48 hrs post transfection. LipofectAMINE Transfection of NIH3T3 Cells 1. Plate 3T3 cells at 2 x 105 per 35 mm dish & let grow overnight (cells should be 50-70% confluent for transfection). 2. For each transfection, dilute: A: 8 μl