赛默飞世尔Thermo Fisher 20 ML LAB F

SYBRGreen Q-PCR in the ABI 7700

Green 1:100 dilution  (50 µL SYBR Green concentrate, 5 mL DMSO, store aliquots at -20ºC in dark tubes in a dessicator) SYBR Green 1:2000 solution (0.5 mL of SYBR Green 1:100 dil.,  1 mL glycerol, 10 uL Tween,  8.5 mL H2 O, store at -20ºC in dark

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15

酵母转化的几种方法

enough of this culture to 300 ml YEPD to produce an OD6oo=0.2 (ca. 20 mls.). Incubate at 30°C to an OD600 of 6.5 to 8.5. I often grow three cultures overnight starting with different amounts of inoculum and use the one at the best OD in the morning. Pellet the cells