赛默飞世尔Thermo Fisher SILICA 100-

Silica Clean-up of DNA

for downstream digests oheat 5 minutes at 60 degrees ospin 2 min @ full speed in microcentrifuge oremove DNA-containing supernatant to clean tube Notes & Misc: •Conveinient PCR clean up before digestion: 100μl PCR reaction cleaned up with 50μl silica

DNA Extraction Protocols Using Silica

hood using PCR-free chemicals and supplies.Materials needed (suggested stocks are sufficient for several hundred "extractions"): Silica - ~100 g, Sigma # S-5631 GuSCN - ~500 g, Amresco (cheaper) # 0380 or Sigma # G-6639 Triton X-100

Silica Clean-up of DNA

Materials: Silica Suspension: add 2 g of silica to 15 ml of H2O wash 3x by centifugation at 2000 x g for 2 min estimate vol of silica