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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311768699
中文名称:
英文名称:X72 SAMPLE BOTLLE SNAP CAP 60ML
货期:热销品现货,其他请咨询




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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min
RNA purification using TRizol--TRIZOL法提取RNA
with 1 ml 75% ethanol. c.Mix sample by vortexing. The RNA pellet may float. d.Centrifuge at 12000 x g for 5 minutes at 4oC. If this RNA will be used for RT-PCR repeat steps a,b and c twice. e.RNA pellet may be stored in ethanol at -70oC for months. 5.
FACS Procedures for Apoptosis Detection
stock 200µg/ml in dH20 (40 ) . 12x75mm snap cap tubes (Falcon 2054). PBS. Controls Required: Cells only (no treatment/no stain) Treated cells with FITC-Annexin V only Treated cells with 7-AAD only Untreated
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