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文献和实验An Ultra-fast method of DNA extraction from Neurospora
-junction. Commercial manifolds are also available. 8. Place each column into a 15 ml conical tube with the tip of the column resting inside a 0.5 ml microfuge tube at the bottom of the 15 ml tube. Add 150 ul of ddH2 0 at 65°C and spin in a tabletop
An Ultra-fast method of DNA extraction from Neurospora
manifolds are also available. 8. Place each column into a 15 ml conical tube with the tip of the column resting inside a 0.5 ml microfuge tube at the bottom of the 15 ml tube. Add 150 ul of ddH2 0 at 65°C and spin in a tabletop centrifuge at maximum
Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh
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