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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311390474
中文名称:
英文名称:X100 GR. 44 24.0CM /100/PKFILTER
货期:热销品现货,其他请咨询








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文献和实验in the stir bar. 13.Bring the vlume up t 1 liter by adding mre f the Lysis buffer. 14.Add Tritn-X100 t a final cncentratin f 2% by vlume. Nte: The Tritn-X-100 will nt disslve immediately.It will g int slutin slwly ver night. 15.Stir gently ver night
. Rinse with distilled water; double distilled water and ethanol. Put 1.5 mm thick spacers on the 20 x 40 cm glass plate, put the notched glass plate on top of the spacers. Clamp the plates together on one side, top and bottom with large binder
RIPA, NP40 lysis buffer, Triton X100 lysis buffer. Use ~750μl/10cm plate (500μl/6cm, 250μl/well of 6-well plate). 2. Spread lysis buffer evenly over cells, let plate sit on ice for 5-10min, then scrape lysate into pre-chilled microfuge tube.
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