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999
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北京泽平
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EA
货号:T_70105-552-0
中文名称:
英文名称:15ML CENTRIFUGE TUBE PS 500/CS
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
, into a sterile 15ml conical centrifuge tube. V. Reagents: Chang media: IRVINE cat# T100-018, liquid and frozen supplement,100ml bottle, store supplement frozen, basal media at 4° C. Colcemid: GIBCO cat# 15210-040. 10µg/ml in Hank's
RNA purification using TRizol--TRIZOL法提取RNA
of chloroform to each tube.b.Cap each tube. Shake samples vigorously by hand for 15 seconds.c.Incubate samples for 5 minutes at room temperature.d.Centrifuge samples for 15 minutes at 12,000 x g at 4oC.3. RNA Precipitation a.Transfer the upper aqueous phase
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