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货号:T_70116-SVA
中文名称:
英文名称:AMBER VIALS,16ML,21X70 200/PK
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
7 mL of 1.15X ligation buffer. 19. Add 400 µL of 20% Triton X-100 (to a final concentration of 1%). Incubate with occasional gentle shaking for 1 h at 37°C. 20. Add 50 µL of T4 DNA ligase. Incubate for 4 h at 16°C
Hints and precautions for the care, feeding and breeding of Neurospora
. 4:21-25). The formula in Davis and de Serres, p. 86, incorporates this change. The trace elements used for Vogel''s medium N are suitable for SC at 0.1 ml per liter final volume; there is no need for a separate formula. Russo, et al. (1985 N.N. 32
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
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