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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Large-scale yeast transformation
. Resuspend yeast in the 90 mls LiAc/TE, put back in same flask (rinsed with sterile water). Shake at 30o C for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30o C for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3 . Cells ready
inhibition of elongation activity and accumulations of C2n:0-ACP (n>4), further indicating that the target of inhibition in S. aureus is the FabF condensing enzyme. References 1. Heath, R. J. & Rock, C. O. Fatty acid biosynthesis as a target for novel
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