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货号：T_70311788769
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英文名称：X1000 OPEN-TOP SEAL 20 MM
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文献和实验, but in only one are the chromosomes separated sufficiently for counting. That spread is magnified in the right-hand image showing 20 chromosomes, the proper diploid karyotype of X. tropicalis . Figure 2: Appearance of X. tropicalis inbred (Nigerian) line
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Tris(PH=6.8) 10% SDS 40 µl 10% ammonium persulfate 40 µl TEMED 7 µl C. Preparation of gel 1. Assemble the glass plates and spacers (0.75 mm thick). 2. Pour the running gel to about 1 cm below the wells of the comb (~3.2 ml). 3. Seal with 1 ml
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