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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310606151
中文名称:
英文名称:X200 Stripettes individual p/p wrap 25mL (case of 200)
货期:热销品现货,其他请咨询








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文献和实验High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
to analyze the levels of 3?nitrotyrosine in specific proteins in a variety of biological samples, including human plasma and sputum. Selective?capture antibodies are used to immunoprecipitate individual proteins from samples onto isolated spots
of individual nucleotides 1:200 in 10 mM Tris pH 7.5) 4 μl random hexamer (1 μg/μl stock = 5X) 5 μl 32 P-dCTP 2 μl 10X Klenow buffer 1 μl Klenow 20 μl (3) Incubate 30 min at 37° C (4) Add TE to increase volume to 50 μl (5) Run sample through spin column
down briefly. Put on ice immediately to cool. (2) To Eppendorf with DNA & H 2 O add: 1 μl each dATP, dGTP, dTTP (dilute 10 mM stocks of individual nucleotides. 1:200 in 10 mM Tris pH 7.5) 4 μl random hexamer (1 μg/μl stock = 5X
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