赛默飞世尔Thermo Fisher BIS(TRI-O-T

v468 chapter 5 糖原合成酶激酶-3 的抑制

for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin

Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.

(100 to 200 µl; 1:400) and incubate for 45 min. 7- Wash twice in TBS-T. 7bis- block endogenous peroxidase by incubating in 0.1NaN3 and 0.3 H2O2 for 30 min. Wash thrice. 8- add the HRP-conjugated avidin (100 to 200 µl, dilution 1:300 -500) (Dako P

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

?diisopropylaminophosphinyl]guanosine with N 2 ?isobutyryl?2??O ?propargyl?3??O ?(levulinyl)guanosine provides, after P (III) oxidation, 3??/5??deprotection, and purification, the 2??O ?propargylated guanylyl(3??5?)guanosine 2?cyanoethyl phosphate triester in a yield of 88