赛默飞世尔Thermo Fisher Fisherbrand

将滋养层和内细胞群分别染色，TUNEL检测牛胚胎凋亡

solution) - Prepare a 2.5 mg/ml stock 1 solution by dissolving PI (Sigma P4170) in PBS and store at 4°C.  On the day of use, dilute the stock 1 solution 1:25 in 0.5% Triton X-100 to give a working concentration of 100 µg/mL.  Discard whatever is not used

高效液相色谱―电喷雾串联质谱法测定水产品中红霉素的残留

； 红霉素内标工作液：准确吸取适量红霉素�13C2标准储备液，用甲醇逐级稀释配成100 μg/L的内标工作液。 　　2.2  样品处理方法 　　2.2.1  制样  鱼，去鳞、去皮，沿脊背取肌肉；虾，去头、去壳，取肌肉部分；河蟹取可食部分；用均质机将其搅碎，并充分混匀。 　　2.2.2  提取和去脂  称取均质样品5．００ g置于50 mL离心管中，加入100 μL内标工作液和15 mL乙腈，立即搅拌、将其分散开，超声提取5 min，震荡提取5 min，以4000 r/min

Stripping Western Blots

. Add 1ml lysis bufferb and vortex briefly. 11) Repeat Step #10 2-3 more times. 12) After last wash, aspirate supernatant and remove remainder of wash buffer with fine gauge (>22g) needle. Process for kinase reaction (as indicated) or add 40-80μl 1X