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文献和实验in an (A) to (T) or a (T) to (A) change in the central (AATT) tetranucleotide sequence. SgrA I, which recognizes and cleaves the sequence CRCCGGYG, displays a new phenomenon of relaxation of sequence specificity. Under standard reaction conditions
Differential cDNA Screening Procedures
2 min. 4.Incubate nitrocellulose discs in denaturation buffer 30 sec, then neutralisation buffer for 30 sec. Standard recipes. 5.Wash filters in 2 x SSPE, rubbing off any adhering agar (This doesn't affect plaque blots). 6.Air dry the filters
Immuno-Laser Capture Microdissection
(A) and immunostained for CD45RO (B). Immunostaining highlights T cells within the germinal center and in the perifollicular area and makes architectural features more evident. Magnification x100. Figs. C & D : Microdissection from germinal centers
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