赛默飞世尔Thermo Fisher TRAY RECTAN

SDS-PAGE

. For the BRL gel box and 0.8 mm thick gels, start the gel at 11 mAmps, and after 1.75 hours (dye should now be into the resolving gel) turn it up to 16 mAmps and run a further 3.15 hours. (Total run takes ~5 hours.) Using a programmable power supply

Preparation of Embryonic Extract

. 9. 25-50 ml Hank's balanced-salt solution, on ice. 10. 12-day chicken embryos, 12. 11. Polytron with PTA 10TS generator or a Dounce homogenizer with a Teflon pestle of coarse fitting. 12. 50 ml conical disposable centrifuge tubes. 13

【转贴】immunofluorescence staining

stock purchased).Wash X 2 @ 5 min. with PEM.Alternately, 0.1 M ammonium chloride diluted in PEM buffer can be used to quench.Incubate for 10 min. Wash X 2 with PEM following this treatment, then 1 X with TBS-T.Counterstain DNA with 1X DAPI diluted in TBS