赛默飞世尔Thermo Fisher X20 VOLUM F

AFFINITY PURIFICATION OF ANTIBODIES

AFFINITY PURIFICATION OF ANTIBODIES (Fisher et al.,1988, Cell 54: 813-822) Conjugation of carrier (BSA) to peptide 1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate, pH 7.0. 2. Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) Stir bars (VWR) 50‐ and 250‐mL round‐bottom flasks (Kontes) Rubber septa for 14/20‐ and 24/40‐glass

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

10‐, 25‐, 50‐, 100‐, 250‐mL round‐bottom flasks (Kontes) 4‐mL screw‐cap glass vials Rubber septa for 14/20‐ and 24