赛默飞世尔Thermo Fisher ZIP BAG 13X

Single Clone Excision Protocol

500ml 20% maltose and 500ml 1 M MgSO4 to 50 ml LB broth). Next day: 3.If you're making new cells,spin them down 5 min at 1500 x g in the C0650 rotor in the Beckman centrifuge,then resuspend in about 40 ml 10 mM MgSO4,and quantify absorbance

Northern Blotting方法(Howell Lab)

for at least 30 min before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml

BAC/PAC 克隆完全攻略,强烈申请加分!!

, the sonicated human placental DNA can be added during the pre-hybridization to the bag/bottle at a level of 100-200 ug/ml of hybridization solution. Solutions: Church Buffer -- 1 Liter BSA 10 gm 0.5M EDTA 2 ml 1 M NaHPO4(pH: 7.2) 500 ml 20% SDS 350 ML