赛默飞世尔Thermo Fisher CHEMWARE BE

常用缓冲液配制－2

to 1 liter store at 4oC Valap* Put 50 g Vaseline, 50 g lanolin, and 50 g paraffin (all available from Fisher) in a 1 L Pyrex beaker Heat on "low" setting on a hotplate, stirring occasionally, until all components are melted and well mixed Pour

Stripping Western Blots

. 4mls/10cm plate), add fresh trypsin/ EDTA (e.g. 1ml/10cm plate), and incubate at 37oC as appropriate to loosen cells (e.g. 1-2min for NIH3T3, 2-5min for MDCK). 3. Add 4 volumes of soybean trypsin inhibitor (1mg/ml in serum-free medium) e.g. 4mls

Prime‐, Stress‐, and Cue‐Induced Reinstatement of Extinguished Drug‐Reinforced Responding in Rats: Cocaine as the Prototypical Drug of Abuse

10‐ml bottle heparin sodium (1000 U/ml concentration) Glycerol USP (Fisher Scientific or similar)