赛默飞世尔Thermo Fisher SHELF FOR O

Procedures for In Vitro Production of Bovine Embryos

Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering

14-826-5D Construct 21G 1 inch needle Fisher Scientific 14-826C Construct

Northern Blotting方法(Howell Lab)

for at least 30 min before quantitation. Pipet up and down several times before taking 2 µl into 998 µl water. Read at 260 nm. RNA concentration (µg/µl) = OD x 500 x 0.04 µg/µl = OD x 20 Formaldehyde gel Boil 2 g agarose in 144 ml