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- 文献和实验
- 技术资料
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999
- 供应商：
北京泽平
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EA
货号：T_70122-048-018
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英文名称：SLIDE CARRIER 30-PL F/STNR EA
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
1 Fork, 1" diameter post Newport PS-F 1 Beam Dump Newport PL15
In Situ HYBRIDIZATION TO TISSUE SECTIONS
will take a bit of practice. - 6-8 micron sections are fairly easily managed - put 1-3 sections on each subbed slide Air dry the slides for at least 20'. Fix with: 4% paraformaldehyde 20' 3X PBS 5' 1X PBS 5' 1X PBS 5' 30% EtOH
In situ hybridisation to alpha satellite sequences (chromosome specific)
can be done without formamide at higher temperature (try 0.25 x SSC at 72C for 5 minutes) Formamide wash solution: 60ml formamide TOXIC (Sigma F7503), 30ml H2 O, 10ml 20 x SSC, 80 m l conc. HCl (to bring the pH to 7) Do not allow the slides to dry at any stage
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