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- T_70114-127-40C
- 2025年07月11日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70114-127-40C
中文名称：
英文名称：IDENTIPLUG 21-26MM 400/PK
货期：热销品现货，其他请咨询
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Native Chromatin Preparation and Illumina/Solexa Library Construction
the reaction by adding MNase stop buffer to a final concentration of 10 mM Tris (pH 7.6), 10 mM EDTA. 25. Sonicate three times for 20 sec each in ice water. 26. Dialyze against 400 mL of RIPA buffer for 2 h at 4°C
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
anti-DNA-POD (1: 5; 1: 10) overnight at 4°C. 7. Wash in PBS (3x5 min). 8. Demonstration of peroxidase activity in a Petri dish: dissolve a DAB tablet (10 mg) in 40 ml of 0.1M citrate acid-sodium citrate buffer (pH 5.5), add 5 ml of H2
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