赛默飞世尔Thermo Fisher WEIGHT TC C

【转贴】immunofluorescence staining

on top of the buffer due to the bubbling action.Note: The NaBH4 should be stored in a dessicator. The final concentration of the NaBH4 in PEM should be 1 mg/ml. It can be difficult to weigh out a specific amount of NaBH4 - it works best to just weight some out, note

Northern Blotting方法(Howell Lab)

bottom twice more with 100 µl buffer each time. Add 750 µl 100% EtOH and let precipitate overnight at -70℃. Spin down at 4℃ for 12 min, dry in the TC hood, and resuspend with DEPC water. RNA quantitation Let resuspended RNA sit on ice

Competitive RT-PCR Strategy for Quantitative Evaluation of the Expression of Tilapia (Oreochromis niloticus) Growth Hormone Receptor Type I

Oligonucleotides Sequences A 5′…(tc)t(ag)aa(ct)tggac(acgt)(tc)t(ag)tt(ag)aa