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999
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北京泽平
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货号:T_70102-225-526
中文名称:
英文名称:WEIGHT TC CLASS 3 5MG
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文献和实验【转贴】immunofluorescence staining
on top of the buffer due to the bubbling action.Note: The NaBH4 should be stored in a dessicator. The final concentration of the NaBH4 in PEM should be 1 mg/ml. It can be difficult to weigh out a specific amount of NaBH4 - it works best to just weight some out, note
Northern Blotting方法(Howell Lab)
bottom twice more with 100 µl buffer each time. Add 750 µl 100% EtOH and let precipitate overnight at -70℃. Spin down at 4℃ for 12 min, dry in the TC hood, and resuspend with DEPC water. RNA quantitation Let resuspended RNA sit on ice
Oligonucleotides Sequences A 5′…(tc)t(ag)aa(ct)tggac(acgt)(tc)t(ag)tt(ag)aa
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