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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70119-068-432
中文名称:
英文名称:POLY LAB COAT LG 40G 30CS
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
SSR GEL and Silver Staining Protocol
. 2. Gels are 6% acrylamide, 8M urea, 1X TBE. For each gel, mix together 50 g urea, 15 ml 40% 19:1 acrylamide solution, and 31 ml d.i. water. We use a 4.5% gel for fingerprinting reactions. Note: (We store aliquots of premixed 40% Acrylogel solution
. 6. Wash membrane(s) 2x for 30 min. each in 0.1% SDS, 65℃ . Discard first wash in liquid waste container. If %26lt;1 mR/h the second wash may be disposed of in the designated lab sink. 7. After last wash, remove membrane(s) and dry the membrane(s) briefly
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