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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
【转贴】immunofluorescence staining
antibody species, e.g., normal goat serum if using Goat anti-mouse IgG), can also be used.Remove blocking buffer and add Primary Antibody diluted in blocking buffer.Incubate either 30 min at 37 C, 1-2 hours at RT, or overnight at 4C. Remove Primary Antibody
substitute (Fisher). - 100% ethanol, 95% ethanol and 80% ethanol. Prepare the 80% ethanol fresh each day. - Phosphate buffered saline (PBS) pH 7.4 - Oxidation blocking solution (PBS + 2% hydrogen peroxide). Make fresh
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