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999
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北京泽平
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货号：T_701AA31572DR
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英文名称：AL23 TUBE (BTH ENDS O 18 200MM
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
Replication timing by density transfer
(until budded cells accumulate). Remove one sample (t = 0), then swirl the flask in ice-water and return it to 23o C. Continue to collect samples (until t = ~80 min). The ice-water treatment is to quick-chill the culture to 23o C, and is usually
Native Chromatin Preparation and Illumina/Solexa Library Construction
is empty. Collect the entire effluent in the same tube (from Step 18). 20. Count cells and check CD4+ T cell purity. Recovery is typically ~20%. Purified CD4+ T cells with ~ 95%-98% purity are used for native chromatin preparation
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