赛默飞世尔Thermo Fisher POT CHLORID

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

) Transcription reaction using chemically synthesized NTPαS, commercial NTPs, and T7 RNA polymerase. Transcribed RNAs were bodily‐labeled by [α‐32 P]ATP during transcription. Transcription reactions are performed with promoter strand and template (1.0 µM

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

. 2010. One‐pot etherification of purine nucleosides and pyrimidines. Org. Lett. 12:4478‐4481.    Lagisetty, P., Russon, L.M., and Lakshman, M.K. 2006. A general synthesis of C6

Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis

1 μl of γ-[32 P]-ATP (CAUTION! see Hint #1) 1 μl of T4 Polynucleotide Kinase enzyme Total volume is 40 μl. 2. Incubate at 37°C for 25 min. 3. Add another 0.5 μl of T4 Polynucleotide Kinase enzyme