赛默飞世尔Thermo Fisher X12 ANALYTI
CAL FILTER FUNNEL0.45µL CN MEMBRANE (PACK OF 12) 货号：T_70310539051- ￥70 - 1400
- Thermo Fisher
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- T_70310539051
- 2025年07月11日
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310539051
中文名称：
英文名称：X12 ANALYTICAL FILTER FUNNEL0.45µL CN MEMBRANE (PACK OF 12)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
10 µl Bio-Rad Kaleidoscope Prestained Standards #161-0324 directly. 3. Run with constant voltage (120V) 4. Usual running time is about 1：40 F. Preparation of membrane 1. Cut a piece of PVDF membrane(Pall Corporation ). 2. Wet for about 30 min
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
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