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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70114-379-094
中文名称:
英文名称:BOTTLE LDPE 500ML PK/12
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文献和实验Cool growth competent E . coli
al., 1990). Chill the following: >100 ml ice-cold TB, centrifuge (GSA rotor), 500 ml centrifuge bottle, and sterile freezing tubes (eppendorf tubes w/ good caps) -Innoculate 250 ml SOB media (2 liter flask) w/ 10-12 colonies bacteria
Procedures for In Vitro Production of Bovine Embryos
Needed 2 x 1 L sterile saline (transport saline) 1 aliquot of stock 16 (Pen-Strep 10 ml) per bottle of saline 3 to 4 bottles of oocyte collection medium (OCM) (400 ml) 1 aliquot of stock 11 (glutamine 4 ml) per bottle of OCM
BP 152-5 (MW 121.14) (890 mM) 110g Boric acid Fisher (electroph.gr) BP 168-500 (MW 61.83) (20 mM) 40 mL 0.5M EDTA pH 8.0 H2O to 2L, check pH w. strip (take out and drizzle ~500 ul on pH strip, ~pH 8.5, filter sterilize 0.2
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