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文献和实验PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS
with clear smooth wall. It should be deep enough to hold the embryo. 4. Keep the plate at 37o C, 5% CO2. Removal of Zona Pellucida Materials: Dissecting microscope; Acid Tyrode's (Sigma: T1788);
PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS
with clear smooth wall. It should be deep enough to hold the embryo. 4. Keep the plate at 37o C, 5% CO2. Removal of Zona Pellucida Materials: Dissecting microscope; Acid Tyrode's (Sigma: T1788);
Procedures for In Vitro Production of Bovine Embryos
)Disposable scalpels #21 (08-927-5D) Dolphin-nose tip microcentrifuge tubes, 1.5 ml (07200210) (Costar 3213) Drummond microdispenser (2117010) (Drummond Sci 3-000-105) Drummond microdispenser replacement tips (Drummond Sci 3-000-105-G) (Fisher
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