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- Thermo Fisher
- 美国/芬兰/中国/韩国/墨西哥
- T_70102-591-29
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70102-591-29
中文名称：低型塑料烧杯,400ml,10/pk
英文名称：BEAKER PP GRAD 400ML 10/PK
货期：热销品现货，其他请咨询
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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
9. After the last centrifugation, discard the supernatant and resuspend PBMCs at 2 x 106 cells/mL in warm (37°C) R10 medium. Aliquot a maximum 107 (or 5 mL) cells per 50-mL PP conical tube and incubate overnight in a humidified atmosphere incubator preset to 37°C
【转贴】immunofluorescence staining
stock purchased).Wash X 2 @ 5 min. with PEM.Alternately, 0.1 M ammonium chloride diluted in PEM buffer can be used to quench.Incubate for 10 min. Wash X 2 with PEM following this treatment, then 1 X with TBS-T.Counterstain DNA with 1X DAPI diluted in TBS
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
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