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999
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北京泽平
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EA
货号：T_70103-410-028
中文名称：
英文名称：1.0ML V-VIAL CLR CRIMP 12/CS
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文献和实验Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
) DMEM/F12 (Invitrogen, Catalog # 12500-096 or equivalent) Materials: BG01V human embryonic stem cells Tissue culture dishes (60 mm; Fisher, Catalog # 08-772B, 100 mm; Fisher, Catalog # 08-772E, or equivalent) 15 mL conical
V294.PART II，Chapter 2 Boyden法体外趋化试验
): dilute 1:10 indistilled H2O before use. 11. Peri dishes. 12. Glass slide and cover glass (32 × 24 mm). 13. Nail polish. 14. Terg-A-Zyme? (cat. no. 1304; Alconox; New York, NY): an enzyme activedetergent available from Fisher. Dissolve
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
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