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Cell Cultures

of an ocular grid inserted into an inverted phase contrast microscope. Cell density (cells/cm2) is then plotted on a log scale against time in culture.Exercise 12.1 - Aseptic Cell Transfers Level I Figure 12.3 Flaming a wire loop and removing cap

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder

Construction and Characterization of Adenovirus Vectors

. For large-scale preparation of Ad vectors using suspension-adapted 293N3S cells: ix. Distribute a 3-L culture of 293N3S cells into eight 500-mL centrifuge bottles. x. Centrifuge at 650g for 20